Full-back Omozusi and midfielder Chambers missed Saturday's 4-0 win over Sheffield Wednesday with hamstring injuries and are unlikely to be ready in time.
Boss Russell Slade will be tempted to name an unchanged line-up following their weekend romp.
On-loan Tottenham teenager Harry Kane started in place of veteran frontman Scott McGleish and marked his full debut with his first senior goal.
Midfielder Matt Sparrow (knee) and striker Jonathan Tehoue (hamstring) were involved despite their recent niggles and came through unscathed.
Romanian forward Adrian Patulea left the club over the weekend after his contract was cancelled by mutual consent.
Source: PA
Source: PA